中国农业科学2012，Vol.45Issue(1)：135-141,7.DOI:10.3864/j.issn.0578-1752.2012.01.016
电子顺磁共振波谱仪分析干红葡萄酒DPPH清除能力及其与总酚含量的关系
EPR Spectroscopy Studies on DPPH Free Radical Scavenging Activity of Dry Red Wines and Their Relationship with Total Polyphenols Content
摘要
Abstract
[Objective] The purpose of this study was to compare the antioxidant capacities of dry red wines and analyze their relationship with total polyphenol content. [Method] The antioxidant properties of five monoverietal dry red wine samples (from the Shacheng viticultural region of China,) were investigated by Electron Paramagnetic Resonance (EPR) spectroscopy using l,l-diphenyl-2-picrylhydrazyl (DPPH) radical as oxidants, and their total polyphenols content were analyzed by FC method. [Result] Generally, the dry red wines which were diluted 200 folds showed high antioxidant in 5 min reaction time, the DPPH scavenging rate of Cabernet was 72.31% which was the highest, Ruby Cabernet 72.23%, Merlot 68.31%, Shiraz 59.03% and Zinfandel 56.34%; the total polyphenols content of Merlot was the highest at 3 044.43 mg·L-1, Ruby Cabernet 3 026.92 mg·L-1, Cabernet 2 886.81 mg·L-1, Shiraz 2 085.51 mg·L-1 and Zinfandel 1 647.64 mg·L-1. [Conclusion] It is an efficient method in detecting the antioxidant capacities of wines by EPR method. The DPPH scanvenging activity from dry red wines showed obviously difference. There was a linear correlation between the antioxidant activities and the total polyphenols content, y=0.01116x+ 37.327665, rGAE/DPPHequiv=0.91753.关键词
干红葡萄酒/抗氧化性/DPPH/总酚/电子顺磁共振(EPR)Key words
dry red wines/antioxidants/DPPH/total polyphenols/EPR引用本文复制引用
胡博然,闻雯,阴淑贞,朱勇,张奉民..电子顺磁共振波谱仪分析干红葡萄酒DPPH清除能力及其与总酚含量的关系[J].中国农业科学,2012,45(1):135-141,7.基金项目
江苏省政府留学奖学金项目(K001)、江苏省高校产业化推进项目(JH08-24) （K001）
