中国农业科学2012，Vol.45Issue(2)：208-217,10.DOI:10.3864/j.issn.0578-1752.2012.02.002
陆地棉胞质雄性不育系与保持系线粒体基因组RFLP分析
RFLP Analysis of Mitochondrial Genomes Between Cytoplasmic Male Sterile Line and Maintainer Line in Upland Cotton
摘要
Abstract
[ Objective 1 Identification of the differences of mitochondrial genomes between cytoplasmic male sterile (CMS) line and maintainer line of cotton could make a foundation for cloning CMS-associated gene in cotton. [Method] In this study, 20 mitochondrial gene probes, atpA, atp6, atp9, ccmB, ccmC, ccmFNl, cob, coxl, coxll, coxIII, matR, nadlbc, nad2, nad4, nad5, nad6, nad7c, nac/9, rplS, and rml8, were used to identify the differences between the P30B normal fertile (N-) and the P30A male sterile (S-) cytoplasm with Southern blot method. [ Result] Among the 20 genes, atpA, atp9, ccmB, nadlbc, nad6, nad7c and rrnl8 revealed restriction fragment length polymorphisms (RFLPs) between N- and S-cytoplasm. The differences in atpA locus were most obvious. [Conclusion] atpA was an important mitochondrial genomic site that showed an obvious difference between CMS line and maintainer line of cotton. One rrnJ8 gene copy was deleted in the mitochondrial genome of CMS line.关键词
棉花/细胞质雄性不育/线粒体基因组/RFLP/atpAKey words
cotton (Gossypium hirsutum L.)/ CMS (cytoplasmic male sterility)/ mitochondrial genome/ RFLP/ atpA引用本文复制引用
张晓,张锐,史计,孟志刚,孙国清,周焘,郭三堆..陆地棉胞质雄性不育系与保持系线粒体基因组RFLP分析[J].中国农业科学,2012,45(2):208-217,10.基金项目
国家自然科学基金资助项目(30771371) （30771371）
