中国农业科学2012，Vol.45Issue(2)：320-329,10.DOI:10.3864/j.issn.0578-1752.2012.02.014
甜樱桃DFR基因内含子2和内含子3的多态性
Polymorphism of DFR Gene Intron 2 and Intron 3 in Sweet Cherry
摘要
Abstract
[Objective] The relationship between polymorphism of DFR gene and pericarp colors was studied in 70 sweet cherry varieties. [Method] DNA sequences analysis was applied to detect the polymorphism of DFR gene in 10 sweet cherry varieties (Prunus avium L.) of different colors in pericarp. Special primers were designed to amplify the polymorphic DNA fragments. 70 sweet cherry varieties (P. Avium L.) were used to test DFR gene polymorphism. [Result] The DNA sequences of partial DFR gene about 1 kb were obtained from sweet cherry (P. Avium). The identity of these DNA sequences was 80% with DFR gene of peach (Prunus persica). The identity of predicted amino acid sequences was 99% with the amino acid sequence of DFR in sweet cherry. This partial DFR gene contained 3 extrons and 3 introns. Two polymorphic loci were detected in intron 2 and intron 3. Three haplotypes and five haplotype combinations were found among 70 sweet cherry varieties which are composed of yellow pericarp group, yellow with a pink blush pericarp group and red pericarp group. There was no significant difference in the DFR gene frequencies between yellow with a pink blush pericarp group and dark red pericarp group. [Conclusion] Two polymorphism loci were detected in intron 2 and intron 3 of DFR gene. Preponderatn alleles frequencies of 70 sweet cherry varieties was 0.864 in intron 2 and 0.679 in intron 3. Respectively. There is no direct relationship between pericarp colors and polymorphisms DFR gene in intron 2 and intron 3.关键词
甜樱桃/DFR基因/果皮颜色/等位基因频率/基因多态性Key words
sweet cherry/ DFR gene/ pericarp color/ allele frequencies/ gene polymorphism引用本文复制引用
王廿,鄢锦辉,张开春,王晶,张晓明,闫国华..甜樱桃DFR基因内含子2和内含子3的多态性[J].中国农业科学,2012,45(2):320-329,10.基金项目
北京市自然科学基金项目(6073026)、北京市科技新星计划(2010B025)、北京市农林科学院科技创新能力建设专项(KJCX201102003)、公益性行业(农业)科研专项经费(200903019) （6073026）
