中国农业科学2012，Vol.45Issue(2)：353-358,6.DOI:10.3864/j.issn.0578-1752.2012.02.018
番鸭就巢性状相关基因表达的cDNA-AFLP分析
cDNA-AFLP Analysis on Transcripts Associated Gene with Broodiness in Muscovy Duck
摘要
Abstract
[Objective] The objective of this experiment is to identify differentially expressed genes of Muscovy Duck broodiness. [ Method ] cDNA-AFLP approach was employed to analyze genes differentially expressed in broodiness and non-broodiness Muscovy Duck individuals derived from a single RF white feathers line. [Result] There were 82 differentially expressed bands associated with broodiness behaviors, 15 bands of them were subject to sequence analysis. Database searches indicate that 8 cDNA fragments show high homology to known genes, which include some function genes in hypothalamic-pituitary-gonadal axis regulating reproduction, such as GnRH gene and FSH gene. Meanwhile genes were also found in GH-IGF axis, such as GH gene. Other genes participated in signal transduction, transcription, metabolism biological processes, such as superoxide dismutase, keratin related protein, thiocyanate enzyme release S-transferase enzymes etc. Two cDNA fragments show homology to unknown function genes, 5 cDNA fragments show no detectable homology to know genes, suggesting that they probably represent novel genes. Three bands selected randomly from them were confirmed by RT-PCR and that result was consistent with the results of cDNA-AFLP. [Conclusion] The results of this paper uncovered the regulation mode of gene express variation of different Muscovy Duck broodiness traits, and thus further providing the molecular mechanism of broodiness of Muscovy Duck.关键词
番鸭/就巢,cDNA-AFLP/表达谱/差异表达基因Key words
Muscovy Duck (Cairina moschata)/ broodiness/ cDNA-AFLP/ gene expression profiling/ differentially expressed genes引用本文复制引用
吴旭,严美姣,刘丽平,傅星源,连森阳,李昂..番鸭就巢性状相关基因表达的cDNA-AFLP分析[J].中国农业科学,2012,45(2):353-358,6.基金项目
国家自然科学基金(31001005)、"十二五"国家水禽产业技术体系专项经费(CARS-43)、福建省自然科学基金(2009J01049)、福建省科技厅青年人才项目 (2008F3006) （31001005）
