中国农业科学2012，Vol.45Issue(3)：548-555,8.DOI:10.3864/j.issn.0578-1752.2012.03.017
ALV-J相关的鸡急性纤维肉瘤发病模型的建立
Establishment of a Bird Experiment Model for Chicken Acute Fibrosarcomas Induced by ALV-J Associated Virus
摘要
Abstract
[Objective] An experiment was carried out to prove that the field chicken fibrosarcoma mass contains acute fibrosarcoma-inducing ALV-J associated virus. [Method] One-day-old 817 hybrid chickens were inoculated through different routes with tumor suspension filtrate containing ALV-J associated virus. One-day-old 817 hybrid chickens and SPF Leghan chickens were inoculated subcutaneously with tumor suspension filtrate containing ALV-J associated virus in different doses. Continuous observation of tumor development was made in 30 days. [Result] In the groups of inoculations through the subcutaneous, in muscule and intra-abdominal, the tumor incidence was 100% (20/20), 95.2% (20/21), and 100% (20/20), respectively. The tumor incidence of 817 hybrids and SPF chickens inoculated with undiluted tumor extract filtrate was 100% (10/10). When birds were inoculated with tumor extracts in 1 : 10 and 1 : 100, tumor rates were still in the range of 80% (8/10) to 100% (10/10) for both chicken lines. When birds were inoculated with tumor extracts in 1 : 1 000, tumor rates decreased to 40% (4/10) or 50% (5/10). No tumor development was observed in 30 days when birds were inoculated with the tumor extract of 1 : 10 000 dilution. However, there were 2/6 chickens developed tumor mass when inoculated with supernatant from tumor extract-infected DF-1 cell culture. Histopathology indicated that the induced tumors were typical fibrosarcoma. [Conclusion] The fibrosarcoma tissues of 817 hybrid chickens of field case contained ALV-J associated acutely tumorigenic virus. Both tumor extract filtrate or extract-infected DF-1 cell culture supernatant could induce acute fibrosarcomas in chickens of different lines, and the tumor started to appear as early as 7 days after inoculation. The dynamics of the fibrosarcoma development and tumor rates were closely related to doses of the tumor extracts in inoculation.关键词
鸡急性纤维肉瘤/ALV-J(J亚群禽白血病病毒)/鸡体致病试验Key words
acute chicken fibrosarcomas/subgroup J avian leukosis virus/bird experiment model引用本文复制引用
李传龙,张恒,赵鹏,崔治中..ALV-J相关的鸡急性纤维肉瘤发病模型的建立[J].中国农业科学,2012,45(3):548-555,8.基金项目
国家自然科学基金面上项目(31172330)、国家公益性行业农业科研专项经费项目(20123055) （31172330）
