中国农业科学2012，Vol.45Issue(3)：562-568,7.DOI:10.3864/j.issn.0578-1752.2012.03.019
亚慢性镉暴露对五指山猪肝脏生化指标的影响
Effect of Sub-chronic Cadmium Exposure on Liver Biochemical Index in Wuzhishan Pig
摘要
Abstract
[Objective] The objective of this study was to look for a sensitive monitoring index of liver injury induced by cadmium and provide a scientific basis for cadmium monitoring and prevention by studying the influences of subchronic cadmium exposure on liver biochemical indexes. [Method] Twenty healthy 3-month-old Wuzhishan Pig (WZSP )were divided randomly into 5 groups, and then fed with basal diet containing 0.0 mg·kg-1 (group Ⅰ ), 0.5 mg·kg-1 (group Ⅱ), 2.0 mg·kg-1 (group Ⅲ), 8.0 mg·kg-1 (group Ⅳ), 32.0 mg·kg-1 (group Ⅴ) doses of cadmium respectively over one hundred days. Blood were collected every 20 days to determine the activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and y-glutamyltransferase (γ-GGT). Blood cadmium and liver cadmium were determined at the end of experiment. [Result] Compared with group Ⅰ , ALT activity in groups Ⅱ and Ⅲ increased significantly after exposing to cadmium for 80 days (P<0.05), followed by 60 days for group Ⅳ and 20 days for group Ⅴ but not within 40 days. AST activities in groups Ⅱ and Ⅲ increased significantly after exposing to cadmium for 20days (P<0.05) but not within 40 days (P>0.05), while groups Ⅳ and Ⅴ increased significantly within 100 days (P<0.05). GGT activities in groups Ⅱ, Ⅳ, and Ⅴ all increased significantly after exposing to cadmium for 100 days (P<0.05). Liver cadmium and blood cadmium in groups Ⅳ and Ⅴ increased significantly compared to group Ⅰ (P<0.05). There was a significant correlation among cadmium exposure, blood cadmium and liver cadmium at the 0.01 level, ALT activity was significantly correlated with cadmium exposure and liver cadmium at the 0.05 level. [Conclusion] Liver injury can be induced by subchronic cadmium exposure at concentrations higher than 0.5 mg·kg-1. Serum ALT activity and blood cadmium can be used respectively as a liver effective biomarker and exposure biomarker of liver injury induced by subchronic cadmium exposure.关键词
五指山猪/镉/肝脏/亚慢性毒性Key words
Wuzhishan Pig/cadmium/liver/subchronic toxicity引用本文复制引用
吴小胜,魏帅,魏益民,郭波莉,杨鸣琦,刘晓玲..亚慢性镉暴露对五指山猪肝脏生化指标的影响[J].中国农业科学,2012,45(3):562-568,7.基金项目
农业部"948"计划项目(2009-Z45(22)) （2009-Z45(22）
