中国全科医学2012，Vol.15Issue(9)：999-1001,3.
三种不同方法诱导3T3-L1脂肪细胞胰岛素抵抗的对比研究
Comparative Study of Inducing Insulin Resistance in 3T3-L1 Adipocytes with Three Different Methods
摘要
Abstract
Objective To observe the glucose consumption and Glut4 expression in inducing insulin resistance in 3T3 - L1 adipocytes with three methods ( dexamethasone, free fatty acids, high glucose and hyperinsulinism ) . Methods Three methods were used to induce insulin resistance in 3T3 - L1 adipocytes, and the glucose consumption and Glut4 expression were detected in each group in 12 h, 24 h, 36 h, 48 h and 60 h. Results Within 24 hours, compared with the normal control group, the glucose consumption in dexamethasone group, free fatty acids group and high glucose and hyperinsulinism group decreased significantly ( P < 0. 05 ) . After 24 hours, compared with the normal control group, the glucose consumption in free fatty acids group showed no statistically significant difference ( P > 0. 05 ) . The glucose consumption in dexamethasone group decreased significantly compared with normal control group ( P < 0. 05 );compared with the dexamethasone group, the glucose consumption in high glucose and hyperinsulinism group decreased significantly ( P < 0. 05 ) . Compared with the normal control group, the Glut4 expression in dexamethasone group and free fatty acids group both decreased significantly ( P < 0. 01 ), and compared with dexamethasone group and free fatty acids group, the Glut4 expression in high glucose and hyperinsulinism group decreased significantly ( P < 0. 01 ) . Conclusion High glucose and hyperinsulinism can induce stronger insulin resistance in 3T3 -L1 adipocytes, and this may result from the inhibition of Glut4 expression.关键词
脂细胞/3T3-L1细胞/胰岛素抵抗/葡萄糖消耗/葡萄糖转运体4型Key words
Adipocytes/3T3-L1 cells/Insulin resistance/Glucose consumption/Glucose transporter type 4分类
医药卫生引用本文复制引用
陈立,杨明炜,库宝庆,莫阔,汪继敏,王晓翠,马聪..三种不同方法诱导3T3-L1脂肪细胞胰岛素抵抗的对比研究[J].中国全科医学,2012,15(9):999-1001,3.基金项目
湖北省卫生厅2004年度中医药中西结合课题(2010.5-2011.5) （2010.5-2011.5）
