中国人兽共患病学报2012，Vol.28Issue(5)：413-417,5.
两株不同毒力EV71全基因组序列测定及分析
Complete genome sequence analysis of two enterovirus 71 strains with different leveel of virulence
摘要
Abstract
In ordor to understand the prevalence and variation of Enterovirus 71 (EV71) in Jinan in 2008, and to seek the potential virulence gene site of EV71, The complete genome of JN200803 and JN200804 strains was amplified, sequenced and analyzed. The results showed that the full length of the EV71 JN200803 and JN200804 genome were 7 405 and 7 404 nt respectively, and no insertion or deletion was detected in the coding region. The complete genome sequences of JN200803 and JN200804 were compared. There were 106 nucleotide variations were founded, induding 98 nucleotide variations in the coding region, 8 nucleotide variations in the noncoding regions. The nucleotide variations happened in the coding region were synonymous mutations except 16 amino acid mutations. The phylogenetic analysis based on VP1 region and complete genome indicated that JN200803 and JN200804 had the closesst genetic relationship with Fuyang/17. 08/1, and belonged to the same subgeno-type C4a. The virulence gene site of EV71 strains isolated from Jinan possesses not only non-singularity but also the special district characteristics of Jinan.关键词
EV71/毒力/全基因组/序列分析Key words
Enterovirus 71/ virulence/ complete genome/ sequence analysis分类
医药卫生引用本文复制引用
李鹏,宋楠楠,岳盈盈,李志会,张颖,盖中涛,孟红..两株不同毒力EV71全基因组序列测定及分析[J].中国人兽共患病学报,2012,28(5):413-417,5.基金项目
国家自然科学基金资助项目(No.81000720)和山东省自然科学基金资助项目(ZR2011HQ030)联合资助 （No.81000720）
