中国人兽共患病学报2012，Vol.28Issue(5)：433-437,441,6.
2010年南京人群甲型H3N2流感分离毒株全基因组特性分析
Analysis on genomic characteristics of H3N2 subtype influenza A virus isolated in Nanjing, 2010
摘要
Abstract
The molecular characteristics and evolutionary features of H3N2 subtype influenza A viruses in 2010 were to be determined. Kight RNA segments of 3 virus were sequenced. The phylogenetic trees were constructed for analysis of evolutionary characteristics, and variations of important amino acid sites of all eleven kinds of protein were analyzed. No reassort-ment was observed in our isolates. Compared with isolate A/Perth/16/2009, average variations number of amino acid site of HA and NA protein antigenic determinants were 5. 7 and 1. 3 respectively. Compared with isolate A/brisheme 10/2997, the a-mino-acid position 375 of PB1 protein in all isolates had varied (S-*-G) and position 25 of PB1-F2 protein in both Nanjing/1655 and Nanjing/1663 were deleted. In addition, position 31 of M2 protein in all three isolates were N, which was considered as one bio-marker of amantadine resistance. In conclusion the amino acid site variations in HA antigenic determinants of three H3N2 isolates probably result in emergence of new epidemic influenza strains. All three isolates were tolerable to amantadine but susceptible to NA inhibitors. What's more, variations of PB1 (position 375) and PB1-F2 (position 25) might represent the future evolutionary direction of H3N2 viruses.关键词
甲型流感病毒/H3N2亚型/进化/分子特征Key words
influenza A virus/ H3N2 subtype/ evolution/ molecular characteristic分类
医药卫生引用本文复制引用
罗鹏飞,祁贤,卫平民,曹尚,李伟,李亮,吴斌,秦圆方,邓斐,崔仑标,焦永军..2010年南京人群甲型H3N2流感分离毒株全基因组特性分析[J].中国人兽共患病学报,2012,28(5):433-437,441,6.基金项目
江苏省自然科学基金(BK2009434)和江苏省医学重点人才基金(RC2011084)综合资助 （BK2009434）
