中国蔬菜Issue(14)：64-68,5.
温敏型核雄性不育系番茄T4育性的研究
Studies on Fertility of Thermo-sensitive Genic Male-sterile Tomato,Lycopersicum esculentum Mill.—T4
摘要
Abstract
Taking thermo-sensitive genie male-sterile tomato Lycopersicum esculentum Mill. T4 and the control First as experimental materials, this paper investigated pollen germination rate,self-breeding rate and fruit seed number, analyzed the protein changes in leaf and anther by Tricine-SDS-PAGE electrophoresis to comprehend fertile conversion of T4. The paper also analyzed the pollen germination rate, self-breeding rate and fruit seed number, protein differences in leaf and anther under fertility and sterility conditions. The results showed that pollen germination rate, self-breeding rate and fruit seed number of T4 were the highest with 28 ^C/IS V, ( day/night ) treatment. There was no significant difference compared with the control First. It indicated that T4 had restored partial fertility. But, with 28 t/24 °C and 28 t/12 X. Treatments the pollen germination rate, self-breeding rate and fruit seed number of T4 had significant differences with the control First, and T4 expressed as sterile line. There were significant differences in leaf protein and anther protein of T4 under different temperature treatments. Under 28 X /24 X and 28 X IY1 X treatments, leaf protein of T4 was significantly different with the control First. But, there was no significant difference between T4 and the control under 28 Xl\9> Xtreatment.关键词
番茄/温敏型/核雄性不育系/Tricine-SDS-PAGE电泳Key words
Tomato/ Thermo-sensitive type/ Male-sterile line/ Tricine-SDS-PAGE elecrophoresis分类
农业科技引用本文复制引用
王先裕,于分弟,梁聪耀,李良劭,黄维娜,杜永臣,刘政国,曾东,潘立凡..温敏型核雄性不育系番茄T4育性的研究[J].中国蔬菜,2011,(14):64-68,5.基金项目
国家科技部国际合作项目(2006DFA33380),广西科技厅广西科学研究与技术开发计划项目(0718007B-38),南宁市青秀区科学技术局科学研究与技术开发计划项目(20080211B),桂林市局科学研究与技术开发计划项目(20100112-1),国家大宗蔬菜产业技术体系桂林综合试验站项目(CARS-25-G-37) （2006DFA33380）
