中国蔬菜Issue(16)：27-33,7.
甘露聚糖酶与番茄果实硬度的关系
Relationship between Mannanase and Firmness of Tomato
摘要
Abstract
LeMan4 gene was cloned from tomato (Lycopersicon esculentum Mill.) 'Micro-Tom' pericarp. And a plant expression vector of antisense LeMan4 was constructed. Genetic transformation to tomato was mediated by EHA105. PCR and Southern blot test were performed according to Npt Ⅱ sequence. Physiological tests were performed on transgenetic tomatoes. This is aimed to study the role of LeMan4 in tomato softening. The results showed that NptⅡ was integrated into tomato genome, mannanase activity of transgenetic tomatoes was inhibited less than 12.2 % of the control, and transgenetic fruits could ripen, but the firmness were higher than the control, indicating that mannanase was indeed relevant to fruit softening, but not an essential factor for fruit ripening.关键词
番茄/LeMan4反义基因/果实软化/植物表达载体/遗传转化Key words
Tomato/Anti-sense gene of LeMan4/Fruit ripening/Plant expression vector/Genetic transformation分类
农业科技引用本文复制引用
张丙秀,李景富,王傲雪,许向阳..甘露聚糖酶与番茄果实硬度的关系[J].中国蔬菜,2011,(16):27-33,7.基金项目
国家自然科学基金项目(30600414),国家“863”项目(2007AA10Z-178),黑龙江省高校科技创新团队(2009td07) （30600414）
