中国水产科学2012，Vol.19Issue(2)：202-210,9.DOI:10.3724/SP.J.1118.2012.00202
应用SSR和SRAP标记构建青虾遗传连锁图谱
Construction of a genetic linkage map for oriental river prawn (Macrobrachium nipponense) using SSR and SRAP markers
摘要
Abstract
The oriental river prawn, Macrobrachium nipponense, is widely distributed throughout China, Japan, Korea, and Burma. This species is of economic importance in China where about 209 000 t are produced annually by intensive culture. However, the economic value of the species has declined significantly in recent years due to genetic retrogression. To facilitate genetic improvements, we constructed a genetic linkage map for oriental river prawn using SRAP and SSR markers and pseudo-testcross mapping. A total of 175 makers including 27 microsa-tellite and 148 SRAP makers were mapped in 53 linkage groups. The number of markers per group ranged from 2 to 8 and thirty-five groups included no less than three markers. The average number of markers per group was 3.3. The length of groups ranged from 6.7 cM to 91.2 cM (Kosambi). The maximum space between adjacent markers was 49.0 cM while the minimum space was 1.4 cM, with an average distance between markers of 13.1 cM. The framework map contained 16 groups with a length of 997.2 cM. The total length of the map was 2 270.5 cM. The average estimated genome size for oriental river prawn was 4 380.6 cM. Based on the estimated genome lengths, the map accounted for 51.83% of the genome. This is the first genetic linkage map of oriental river prawn. Our data may be used to aid quantitative trait loci (QTL) mapping, gene cloning, and genetic selection. The map also provides a basis for the construction of a higher density genetic linkage map for oriental river prawn.关键词
青虾/日本沼虾/SSR/SRAP/遗传连锁图谱Key words
oriental river prawn/ Macrobrachium nipponense/ SSR/ SRAP/ genetic linkage map分类
农业科技引用本文复制引用
乔慧,吴滟,傅洪拓,龚永生,蒋速飞,熊贻伟..应用SSR和SRAP标记构建青虾遗传连锁图谱[J].中国水产科学,2012,19(2):202-210,9.基金项目
江苏省高技术研究项目(BG2007328) （BG2007328）
中央级公益性科研院所基本科研业务费专项资金资助项目(2011JBFA02). （2011JBFA02）
