中国水稻科学2012，Vol.26Issue(1)：5-8,4.DOI:10.3969/j.issn.1001-7216.2012.01.002
OsBTF3过量表达和RNAi转基因水稻抗盐和抗低温胁迫鉴定
Resistance to High Salt and Cold Stress of Transgenic Rice Seedlings with Over-expressed and RNAi-silenced OsBTF3
摘要
Abstract
To demonstrate the biological function of UsBTF3 gene in rice resistance or tolerance to salt and cold stress, the gene transcription of OsBTF3 responsive to salt and cold treatments was quantified via real-time PCR assay, and resistance to the stress of transgenic rice seedlings with over-expressed and RNAi-silenced OsBTF3 was analyzed. The results showed that gene expression of OsBTF3 was significantly decreased in rice plants under salt stress, while lightly reduced under cold stress. The resistance to salt and cold stress was enhanced highly in the seedlings of transgenic lines with over-expressed OsBTF3, while decreased in the RNAi-silenced 0sBTF3 lines. The results revealed that OsBTF3 might function as a key regulator for abiotic stress resistance in rice.关键词
OsBTF3/基因表达/抗逆境胁迫Key words
OsBTF3/ gene expression/ abiotic stress分类
生物科学引用本文复制引用
李广旭,陈华民,吴茂森,何晨阳..OsBTF3过量表达和RNAi转基因水稻抗盐和抗低温胁迫鉴定[J].中国水稻科学,2012,26(1):5-8,4.基金项目
国家转基因生物新品种培育重大专项资助项目(2009ZX08009-044B,2011ZX08001-002). （2009ZX08009-044B,2011ZX08001-002）
