中国水稻科学2012，Vol.26Issue(1)：9-15,7.DOI:10.3969/j.issn.1001-7216.2012.01.003
水稻黑条矮缩病毒基因组S7编码的2个非结构蛋白在病株中的表达检测
Detection of Two Nonstructural Proteins Encoded by Genome Segment S7 of Rice Black-Streaked Dwarf Virus in Infected Rice Plants
摘要
Abstract
The genome segment S7 of Rite black-streaked dwarf virus (RBSDV) contains two non-overlapping major open reading frames (OKFs), which encoded two proteins. P7a and p7b. Respectively. Based on the sequence of S7 (EU111804), two pairs of S7-specific primers were designed and respectively used to amplify the fragments encoding the p7a and p7b proteins. The expected products were sub-cloned into the expression vector pET-32a( + ) or pSBET and expressed at high level in E. coli BL21 (DE3)pI.ysS or BL21(DE3)pLysE. Respectively. An antiserum specifically against p7a or p7b proteins was prepared using the purified expression proteins to immunize mice. The results of Western blot revealed that both p7a and p7b proteins were expressed in infected plants, and p7a protein was more abundant and easily detected than p7b protein. However, both have not been detected in the viral particles, indicating that both proteins encoded by RBSDV S7 were nonstructural proteins.关键词
水稻黑条矮缩病毒/非结构蛋白/原核表达Key words
Rice black-streaked dwarf virus/ non-structural protein/ prokaryotic expression分类
生物科学引用本文复制引用
吕明芳,羊健,张恒木,陈剑平..水稻黑条矮缩病毒基因组S7编码的2个非结构蛋白在病株中的表达检测[J].中国水稻科学,2012,26(1):9-15,7.基金项目
国家973计划资助项目(2010CB126203) （2010CB126203）
浙江省自然科学基金资助项目(Y3090657). （Y3090657）
