中国水稻科学2012，Vol.26Issue(1)：21-26,6.DOI:10.3969/j.issn.1001-7216.2012.01.005
抗感吡虫啉褐飞虱种群抑制消减杂交cDNA文库的构建
Construction of Suppression Subtractive Hybridization cDNA Library of Imidacloprid-resistant and Imidacloprid-susceptible Brown Planthopper
摘要
Abstract
The forward and reverse subtracted cDNA libraries of imidacloprid-resistant and imidacloprid-susceptible brown planthopper (Nilaparvata lumens Stal) populations were constructed by suppression subtractive hybridization (SSH), and the relative genes regulated by imidacloprid were cloned. The results showed that 120 positive clones obtained represented 92 different single genes, in which 17 shared higher homology with known protein genes of animals in the GenBank. These single genes involved signal transduction, biotic and abiotic stress, reproduction, and etc.关键词
吡虫啉/褐飞虱/抑制消减杂交/基因克隆Key words
imidacloprid/ Nilaparvata lugens/ suppression subtractive hybridization/ gene clone分类
农业科技引用本文复制引用
张珏锋,何月平,陈建明,陈列忠..抗感吡虫啉褐飞虱种群抑制消减杂交cDNA文库的构建[J].中国水稻科学,2012,26(1):21-26,6.基金项目
国家自然科学基金资助项目(30771411) （30771411）
浙江省自然科学基金资助项目(Y307125) （Y307125）
国家科技支撑计划资助项目(2006-BAD08A04-03) （2006-BAD08A04-03）
浙江省科技创新团队资助项目(2010R50028). （2010R50028）
