中国水稻科学2012，Vol.26Issue(1)：70-76,7.DOI:10.3969/j.issn.1001-7216.2012.01.012
利用SRAP分子标记分析47份杂草稻样品遗传多样性
Genetic Diversity of 47 Weedy Rice with SRAP Markers
摘要
Abstract
Genetic diversity of 47 weedy rice accessions collected from disaster-prone areas were analyzed with SRAP molecular markers to further reveal the source of weedy rice based on the genetic background of weedy rice at the level of open reading frame. Among 845 DNA bands amplified with 110 pairs of primers 590 were polymorphic, 5. 4 bands per pair of primers, accounting for 69. 82%. This implied that with a high amplification efficiency at the gene coding region SRAP molecular marker was suitable for genetic diversity analysis. Shannon-Weaver information index of weedy rice in China was 0. 63, the diversity index ( I) of weedy rice from the north and the south of China were 0. 62 and 0. 63. On the basis of genetic distance cluster analysis, significant genetic differentiation was detected between weedy rice from the north and the south. Cheng s index demonstrated that weedy rice from the south of China could be classified as indica rice and weedy rice from the south as japonica rice.关键词
杂草稻/开放式阅读框/相关序列扩增多态性/遗传多样性Key words
weedy rice/ open reading frame/ sequence-related amplified polymorphisms genetic diversity分类
农业科技引用本文复制引用
刘丹,孙健,马殿荣,王嘉宇,唐亮,苗微,陈温福..利用SRAP分子标记分析47份杂草稻样品遗传多样性[J].中国水稻科学,2012,26(1):70-76,7.基金项目
国家自然科学基金资助项目(30671262) （30671262）
辽宁省自然科学基金资助项目(20102192) （20102192）
水稻生物学国家重点实验室开放课题资助项目(090404). （090404）
