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Dose-dependent and combined effects of N-methyl- D-aspartate receptor antagonist MK-801 and nitric oxide synthase inhibitor nitro-L-arginine on the survival of retinal ganglion cells in adult hamsters

Yaoyu Li An'an Yang Tingting Zhu Zhao Liu Siwei You Kwok-Fai So

中国神经再生研究（英文版）2012，Vol.7Issue(10)：725-730,6.

中国神经再生研究（英文版）2012，Vol.7Issue(10)：725-730,6.DOI:10.3969/j.issn.1673-5374.2012.10.001

Dose-dependent and combined effects of N-methyl- D-aspartate receptor antagonist MK-801 and nitric oxide synthase inhibitor nitro-L-arginine on the survival of retinal ganglion cells in adult hamsters

Yaoyu Li ¹An'an Yang ²Tingting Zhu ¹Zhao Liu ²Siwei You ²Kwok-Fai So³

作者信息

1. Department of Ophthalmology, General Hospital of Beijing Military Region, Beijing 100700, China
2. Institute of Neurosciences, the Fourth Military Medical University, Xi'an 710032, Shaanxi Province, China
3. Department of Anatomy, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong Special Administrative Region, China
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摘要

Abstract

This study investigated the effects of daily intraperitoneal injections of N-methyl-D-aspartate receptor antagonist MK-801 and nitric oxide synthase inhibitor nitro-L-arginine (L-NA) on the survival of retinal ganglion cells (RGCs) at 1 and 2 weeks after unilateral optic nerve transection in adult hamsters. The left optic nerves of all animals were transected intraorbitally 1 mm from the optic disc and RGCs were retrogradely labeled with Fluorogold before they received different daily dosages of single MK-801 or L-NA as well as daily combinational treatments of these two chemicals. All experimental and control animals survived for 1 or 2 weeks after optic nerve transection. Our results revealed that the mean numbers of surviving RGCs increased and then decreased when the dosage of MK-801 (1.0, 3.0 and 4.5 mg/kg) and L-NA (1.5, 3.0, 4.5 and 6.0 mg/kg) increased at both 1 and 2 weeks survival time points. Daily combinational use of 1.0 mg/kg MK-801 and 1.5 mg/kg L-NA lead to a highest RGC number that was even higher than the sum of the RGC numbers in 1.0 mg/kg MK-801 and 1.5 mg/kg L-NA subgroups at 2 weeks. These findings indicated that both MK-801 and L-NA can protect axotomized RGCs in a dose-dependent manner and combinational treatment of these chemicals possesses a potentiative and protective effect.

关键词

retinal ganglion cells/optic nerve transection/N-methyl-D-aspartate receptor/nitric oxide synthase/neuronal survival/hamster

Key words

retinal ganglion cells/optic nerve transection/N-methyl-D-aspartate receptor/nitric oxide synthase/neuronal survival/hamster

引用本文复制引用

Yaoyu Li,An'an Yang,Tingting Zhu,Zhao Liu,Siwei You,Kwok-Fai So..Dose-dependent and combined effects of N-methyl- D-aspartate receptor antagonist MK-801 and nitric oxide synthase inhibitor nitro-L-arginine on the survival of retinal ganglion cells in adult hamsters[J].中国神经再生研究（英文版）,2012,7(10):725-730,6.

基金项目

This research work was supported by research grants from Chinese National Key Project for Basic Research,No.2011CB504402 and the National Natural Science Foundation of China,No.30901649 and 30872829. （）

中国神经再生研究（英文版）

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ISSN：1673-5374

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