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Expression profiles of microRNAs after focal cerebral ischemia/reperfusion injury in rats

Fengguo Zhai Xiuping Zhang Yue Guan Xudong Yang Yang Li Gaochen Song Lixin Guan

中国神经再生研究（英文版）2012，Vol.7Issue(12)：917-923,7.

中国神经再生研究（英文版）2012，Vol.7Issue(12)：917-923,7.DOI:10.3969/j.issn.1673-5374.2012.12.007

Expression profiles of microRNAs after focal cerebral ischemia/reperfusion injury in rats

Fengguo Zhai ¹Xiuping Zhang ¹Yue Guan ²Xudong Yang ¹Yang Li ³Gaochen Song ⁴Lixin Guan¹

作者信息

1. Department of Pharmacology, Mudanjiang Medical University, Mudanjiang 157011, Heilongjiang Province, China
2. Department of Endocrinology, Mudanjiang Medical University, Mudanjiang 157011, Heilongjiang Province, China
3. Department of Thoracic Surgery, Mudanjiang Medical University, Mudanjiang 157011, Heilongjiang Province, China
4. Department of Biochemistry, Mudanjiang Medical University, Mudanjiang 157011, Heilongjiang Province, China
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摘要

Abstract

Rat models of focal cerebral ischemia/reperfusion injury were established by occlusion of the middle cerebral artery. Microarray analysis showed that 24 hours after cerebral ischemia, there were nine up-regulated and 27 down-regulated microRNA genes in cortical tissue. Bioinformatic analysis showed that bcl-2 was the target gene of microRNA-384-5p and microRNA-494, and caspase-3 was the target gene of microRNA-129, microRNA-320 and microRNA-326. Real-time PCR and western blot analyses showed that 24 hours after cerebral ischemia, bcl-2 mRNA and protein levels in brain tissue were significantly decreased, while caspase-3 mRNA and protein levels were significantly increased. This suggests that following cerebral ischemia, differentially expressed microRNA-384-5p, microRNA-494, microRNA-320, microRNA-129 and microRNA-326 can regulate bcl-2 and caspase-3 expression in brain tissue.

关键词

focal cerebral ischemia/microRNA/apoptosis/bcl-2/caspase-3/neural regeneration

Key words

focal cerebral ischemia/microRNA/apoptosis/bcl-2/caspase-3/neural regeneration

引用本文复制引用

Fengguo Zhai,Xiuping Zhang,Yue Guan,Xudong Yang,Yang Li,Gaochen Song,Lixin Guan..Expression profiles of microRNAs after focal cerebral ischemia/reperfusion injury in rats[J].中国神经再生研究（英文版）,2012,7(12):917-923,7.

基金项目

The project was financially supported by the National Natural Science Foundation of China,No.30973897 （）

the Natural Science Foundation of Heilongjiang Province,No.D200978 （）

the Postgraduate Innovative Scientific Research Foundation of Heilongjiang Provin （）

中国神经再生研究（英文版）

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ISSN：1673-5374

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