首页|期刊导航|中国神经再生研究（英文版）|Gene expression by simian virus 40 large T antigen-induced medulloblastomas in mice
中国神经再生研究（英文版）2012，Vol.7Issue(12)：932-937,6.DOI:10.3969/j.issn.1673-5374.2012.12.009
Gene expression by simian virus 40 large T antigen-induced medulloblastomas in mice
Gene expression by simian virus 40 large T antigen-induced medulloblastomas in mice
摘要
Abstract
Signaling pathways known to have components with mutations in human medulloblastoma include sonic hedgehog, Wnt/beta-catenin and insulin-like growth factor. Microarray analysis was applied to examine the gene expression changes in medulloblastomas of pTet-on/pTRE-SV40Tag transgenic mice. Altogether, 14 112 genes were detectable, including 152 genes with significantly different expression levels. These genes were associated with immunity, the cell cycle, signal transduction, cytoskeleton and metabolism. To further confirm the microarray data, real-time polymerase chain reactions were used to examine the expression changes of genes related to sonic hedgehog, Wnt/beta-catenin and insulin-like growth factor signal pathways. Immunohistochemistry detected insulin receptor substrate-1 in the nuclei of brain tumor tissue cells from pTet-on/pTRE-SV40Tag transgenic mice, suggesting that SV40 large T antigen may activate the insulin-like growth factor signal pathway to promote tumorigenesis.关键词
SV40 large T antigen/medulloblastoma/cDNA microarray/real-time polymerase chain reaction/insulin-like growth factorKey words
SV40 large T antigen/medulloblastoma/cDNA microarray/real-time polymerase chain reaction/insulin-like growth factor引用本文复制引用
Xiaoluan Wei,Jie Feng,Yinghe Hu..Gene expression by simian virus 40 large T antigen-induced medulloblastomas in mice[J].中国神经再生研究（英文版）,2012,7(12):932-937,6.基金项目
This work was supported by the National Natural Science Foundation of China,No.31000574 （）
the Fundamental Research Fund for the Central Universities,No.78210042 （）
and the Development Program of China during the 10th Five-Year Plan Period,No.2001BA7011 （）
