中国实验动物学报2012，Vol.20Issue(1)：5-9,5.DOI:10.3969/j.issn.1005-4847.2012.01.002
黑线仓鼠及其白化突变系酪氨酸酶基因的克隆与序列分析
Cloning and sequence analysis of tyrosinase gene in Cricetulus barabensis and albino mutant Cricetulus barabensis
摘要
Abstract
Objective In order to reveal the molecular mechanism of the albino mutant Cricetulus barabensis, the tyrosinase gene of Cricetulus barabensis and the albino mutant Cricetulus barabensis was cloned, sequenced, and comparatively analyzed. Methods The Tyrosinase cDNA was amplified from the total RNA of the skin of Cricetulus barabensis and its albino mutant Cricetulus barabensis by reverse transcriptase (RT-PCR). According to the conservative region of tyrosinase gene of mice and rats, three pairs of primers were designed to link three fragments to a long epitope by overlap extension. Then the three fragments were amplified and sequenced and finally spliced into the complete sequence. The mutation site was identified through DNA matching software. Results The tyrosinase genes of Cricetulus barabensis and the albino mutant Cricetulus barabensis were cloned and the mutation site was measured by DNA sequence analysis software. The results showed that they have the same coding region and there is no mutation. Conclusions The reason of the albino trait of the albino mutant Cricetulus barabensis is different from that of albino trait known in mice, not by the mutation of tyrosinase gene coding region, and reqires further investigation.关键词
黑线仓鼠白化突变系/酪氨酸酶基因/克隆/序列比较Key words
Albino mutant Cricetulus barabensis/Tyrosinase gene/Cloning/Sequence Analysis分类
生物科学引用本文复制引用
赵彦斌,孙兆增,叶华虎,王冬平,胡仲明,曾林..黑线仓鼠及其白化突变系酪氨酸酶基因的克隆与序列分析[J].中国实验动物学报,2012,20(1):5-9,5.基金项目
国家自然科学基金资助项目(项目批准号30870347). （项目批准号30870347）
