中国实验动物学报2012，Vol.20Issue(1)：30-33,4.DOI:10.3969/j.issn.1005-4847.2012.01.007
气雾攻击法感染结核病小鼠模型的建立及其综合评价
Establishment and comprehensive evaluation of a mouse model of tuberculosis induced by aerosol infection
摘要
Abstract
Objective To establish a mouse model of tuberculosis by simulating natural infection and make a comprehensive evaluation to the pathological changes of this model. Methods C57BL/6J mice were infected with Mycobacterium tuberculosis H37Rv by aerosol inhalation. At 4th, 6th and 8th week after infection, the infected mice were scanned by micro-CT and the lungs and spleens were taken for pathological examination (HE and acid-fast staining) and colony counting. Results The deterioration of lung infection in mice were indicated by visual observation and micro-CT scanning results, and diffuse infection throughout the whole lungs at 8th week. The presence of granulomatous consolidation and Mycobacterium tuberculosis in the lungs were revealed by HE and acid-fast staining. Conclusions The mouse model of tuberculosis has been successfully established according to the comprehensive analysis by gross inspection, histopathology, imaging and colony counting. Although this mouse model could not completely represent the same situation of tuberculosis in humans, it may find reasonable application and promote researches relevant to tuberculosis.关键词
气雾攻击法/结核病/小鼠模型/Micro-CT扫描/综合评价Key words
Tuberculosis/Aerosol inhalation/Mouse model/Micro-CT/Comprehensive evaluation分类
生物科学引用本文复制引用
刘芳,杨华,周文江,秦波音,范小勇,王德成..气雾攻击法感染结核病小鼠模型的建立及其综合评价[J].中国实验动物学报,2012,20(1):30-33,4.基金项目
上海市科委资助项目(09140902700 （）
11140903001). （）
