中国实验动物学报2012，Vol.20Issue(1)：34-37,4.DOI:10.3969/j.issn.1005-4847.2012.01.008
线粒体钾通道Kcna3基因敲除小鼠初步制备
Preparation of a mitochondrial K + channel Kcna 3 knockout mouse model
摘要
Abstract
Objective Kcna3 is one of mitochondrial K + channels, which may be associated with apoptosis of myocardium during heart ischemia/reperfusion injury. The aim of this study was to establish a Kcnai gene knockout mouse model to study the impact of mitochondrial potassium channel in heart ischemia/reperfusion injury, and explore its relationship with heart failure. Methods A homologous recombination vector was constructed with BAC vector. Strain 129 mouse embryonic stem (ES) cells were targeted knockout of Kcnai by the homologous recombination vector, and screened with G418 plus GANC. The Kcna3-knockout embryonic stem cells were microinjected into blastula of C57BL/6J mice after superovulation. F1 hybrid mice were bred to obtain mouse aggregation chimeras, and were identified by PCR plus sequencing of tail genomic DNA. Results Eight Kcna3+/- mice were harvested and identified from 40 gray mice. Conclusions The heterozygous Kcnai -knockout mouse model is successfully established and it lays a foundation for further breed homozygous mice. It is a good model for study of the relationship between mitochondrial k+ channel abnormality with heart failure, and is of importance in research of drug screening.关键词
钾通道/基因打靶/胚胎干细胞/转基因小鼠/线粒体Key words
Potassium channel, Gene-target, Embryonic stem cells, Transgenic mice, Mitochondria. Mice分类
生物科学引用本文复制引用
张良平,韩俊毅,范慧敏,刘中民,陈炳官..线粒体钾通道Kcna3基因敲除小鼠初步制备[J].中国实验动物学报,2012,20(1):34-37,4.基金项目
上海市科委科研基金(项目编号:064119504) （项目编号:064119504）
上海市浦东新区重点学科群建设项目(项目号:Pkzxkq2010-01) （项目号:Pkzxkq2010-01）
上海市科委基础重点科研基金(项目号:10JC1413400)资助. （项目号:10JC1413400）
