中国实验动物学报2012，Vol.20Issue(1)：76-80,5.DOI:10.3969/j.issn.1005-4847.2012.01.016
三种啮齿类动物非酒精性脂肪肝形成及机制探讨
Formation and mechanism of non-alcoholic fat liver disease in three species of rodents
摘要
Abstract
Objective To evaluate the formation and mechanism of non-alcoholic fat liver disease ( NAFLD ) in three species of rodents by analyzing effect of species differences on replication of NAFLD models. Methods Twenty SD rats, 20 ICR mice and 20 Mongolian gerbils were assigned randomly into 2 groups respectively: model group (fed with high fat diet) and control group (fed with normal diet). After 16 weeks, hepatic pathology was observed with HE and Mallory's trichrome staining, and serum lipid levels (CHO, TG, LDL-c, HDL-c) and liver function (GOP, GPT) , liver index, antioxidant enzyme activity (SOD, GSH-PX, CAT) , hydroxyproline, free fatty acid, MDA of liver were detected. Results Compared with the control group, Hyp, CHO, TG, LDL-c, HDL-c, liver index, GOP, GPT, MDA, FFA were significantly increased, whereas SOD, GSH-PX, CAT were significantly decreased in Mongolian gerbils ( P < 0. 05 , P < 0. 01) ; pathology showed hepatic fibrosis; CHO, liver index, GOP, GPT, FFA, SOD were significantly increased, whereas MDA, GSH-PX, CAT were significantly decreased in SD rats (P < 0. 05 , P < 0. 01) , and formed focal non-alcoholic steatohepatitis; CHO, LDL-c, HDL-c, liver index, CAT were significantly increased, whereas MDA was significantly decreased in ICR mice ( P < 0. 05 , P < 0. 01) , and the liver histology was normal. Conclusions There are obviously statistical differences in lipid metabolism, liver function and oxidative stress among the three species of rodents. They form different NAFLD models: Mongolian gerbils form liver fibrosis model with high TG, CHO, SD; rats form focal fatty hepatitis with high CHO; and ICR mice form hypercholesterolemia model without pathological changes in the liver.关键词
长爪沙鼠/大鼠/小鼠/非酒精性脂肪肝/肝纤维化Key words
Mongolian gerbil/Rat/Mouse/non-alcoholic fat liver disease/Liver fibrosis分类
生物科学引用本文复制引用
李巍,石巧娟,郭红刚,楼琦,卢领群,萨晓婴..三种啮齿类动物非酒精性脂肪肝形成及机制探讨[J].中国实验动物学报,2012,20(1):76-80,5.基金项目
"十一五"科技支撑计划重点项目(2009 BAI83 B02),浙江省实验动物与安全性评价重点实验室(2008F3021). （2009 BAI83 B02）
