中国实验动物学报2012，Vol.20Issue(1)：88-90,3.DOI:10.3969/j.issn.1005-4847.2012.01.019
百草枯致小鼠肺间质纤维化过程中Smad3蛋白的表达
Expression of Smad3 protein during the pulmonary fibrosis induced by paraquat in mice
摘要
Abstract
Objective The expression of Smad3 protein in the pulmonary fibrosis induced by paraquat (PQ) was detected by immunohistochemistry. Methods 57BL/6J male mice (n = 58)were divided randomly into the experimental group and control group. Pulmonary fibrosis was induced by intraperitoneal injection of PQ(10 mg/kg)in the experimental group(n = 48) , while physiological saline was used in the control group(n = 10) by the same method. The mice were sacrificed on days 2,5,7 and 14 in the experimental group and the control ones on day 7. The lungs were taken for histo-logical examinatioon using HE staining and the expression of Smad3 protein was determined by immunohistochemistry. Results The histological changes including edema, hemorrhage and inflammatory cell infiltration were seen in the lung tissues of mice after PQ administration on days 2 and 5. There were slight collagen deposition and plaque-like fibrosis on day 7 and apparent fibrosis on day 14. The Smad3-positive signs appeared in the nucli of infiltrating macrophages and many type II alveolar epithelial cells on days 2, 5 and 7. The positive expression of Smad3 proteins was positively correlated with the amount of infiltrating macrophages. On day 14 the faint Smad3-positive expression was detected in the nuclei of fibroblasts in hyperplastic foci. Conclusions The expression of Smad3 protein is abnormal during pulmonary fibrosis induced by PQ and Smad3 plays an important role in the development of pulmonary fibrosis.关键词
百草枯/中毒/肺间质纤维化/Smad3/免疫组化/小鼠Key words
Paraquat/Intoxication/Pulmonary fibrosis/Smad3 /Immunohistochemistry/mice分类
生物科学引用本文复制引用
董雪松,刘伟,刘淑英,刘志..百草枯致小鼠肺间质纤维化过程中Smad3蛋白的表达[J].中国实验动物学报,2012,20(1):88-90,3.基金项目
辽宁省教育厅基金资助项目(2008785)高等学校博士学科点专项科研基金课题(20092104110001). （2008785）
