中国比较医学杂志2012，Vol.22Issue(2)：1-5,5.DOI:10.3969/j.issn.1671-7856.2012.02.001
不同品系小鼠感染甲型流感病毒肺小血管内血栓形成
Microvascular Thrombosis in the Lung of Different Strains of Mice Infected with Influenza A Virus
摘要
Abstract
Objective To investigate thrombosis in mouse lung tissue after infection with influenza A virus, and compare the differences between mouse strains with different immune status. Methods Influenza H1N1 ( A/California/7/ 2009) and H3N2( A/Brisbane/10/07) were used in this infectivity study. Forty BALB/c mice, 50 NOD-Scid mice, 33 Scid mice, 33 BALB/C-nu mice, 33 NOD/LTJ mice and 10 icosl-KO mice were anesthetized by ether inhalation and inoculated intranasally with 0. 05 mL suspension of infectious virus H1N1 or H3N2. The pathogenic changes were observed and the virus copy numbers in the lung tissue were detected by real-time PCR. Results Varying degrees of interstitial pneumonia were observed in the lung tissue of mice infected with H1N1 and H3N2 viruses. There was thrombosis within the lung tissue in 13 mice of H1N1 group and 6 mice of H3N2 group. The main components of the thrombus were cellulose and platelets. Conclusions Our results support that thrombosis may occur in the lungs of all studied mouse strains after infection with influenza A (H1N1) and (H3N2) viruses.关键词
H1N1/H3N2/静脉血栓/NOD-Scid小鼠/Scid小鼠/NOD/LtJ小鼠Key words
Influenza H1N1 virus/ Influenza H3N2 virus/ Thrombosis/ NOD-Scid mouse/ Scid mouse/ NOD/LTJ mouse/ NOD/LTJ mouse/ icosl-KO mouse分类
医药卫生引用本文复制引用
刘颖,徐艳峰,邓巍,朱华,黄澜,马春梅,鲍琳琳,秦川..不同品系小鼠感染甲型流感病毒肺小血管内血栓形成[J].中国比较医学杂志,2012,22(2):1-5,5.基金项目
中国医学科学院医学实验动物研究所基本科研业务费:DWS200904 （）
北京市科委项目:Z09000700090904 （）
中医药行业科研专项:200907001-5. （）
