中国畜牧兽医2012，Vol.39Issue(1)：23-27,5.
宇佐美曲霉木聚糖酶在哺乳动物细胞中的分泌表达
Aspergillus usamii Xylanase Secrete Expression in Mammalian Cells
摘要
Abstract
The mature peptide coding sequence of xylanase gene xyn (555 bp) was amplified by RT-PCR from Aspergillus niger GIM 3. 36 total RNA extracts. It was subcloned into different locations of the eukaryotic expressing plasmid vector pcD-NA6/His? A. The recombinant plasmid pcDNA-spna and pcDNA-spnb was identified by PCR. Enzyme digestion and DNA sequencing. The result showed that the recombinant plasmids were constructed correctly. Meanwhile, the PK15 cells were transfected with pcDNA-spna and pcDNA-spnb by cationic liposome, and the mRNA of the target gene was determined by RT-PCR. The maximum yield of the recombinant xylanase (transfected pcDNA-spna) in cell culture medium was 8. 53 U/mL and higher 24% the recombinant xylanase 6. 87 U/mL (transfected pcDNA-spnb). To achieve the purpose that microbiology secrete expression in mammalian cells and provided basis for using xyn gene to transgenic.关键词
宇佐美曲霉/木聚糖酶/哺乳动物细胞/分泌表达Key words
Aspergillus usamii/xylanase/mammalian cells/secrete expression分类
生物科学引用本文复制引用
张茂,邹娴,林纯,刘德武,吴珍芳,蔡更元..宇佐美曲霉木聚糖酶在哺乳动物细胞中的分泌表达[J].中国畜牧兽医,2012,39(1):23-27,5.基金项目
国家重大专项(2008ZX08006004、2009ZX08006-012B). （2008ZX08006004、2009ZX08006-012B）
