中国畜牧兽医2012，Vol.39Issue(1)：127-131,5.
猪孤雌激活早期胚胎线粒体分布及mtDNA拷贝数变化的研究
Mitochondrial Distribution and mtDNA Copy Number of Porcine Parthenogenetic Embryos
摘要
Abstract
Mito-Tracker Green and Real-time quantitative PCR were used to respectively detect the mitochondria distribution and mtDNA copy number of porcine parthenogenetic oocytes. The results showed that the distribution of mitochondria was uniform and dense in the every blastomere beginning from 2-cell embryo. The outside of the space in blastomeres was no distribution of mitochondria. Until the blastocyst formation, mitochondria were distributed in blastomeres. mtDNA copy number of 4-cell embryos was significantly higher than that of 8-cell embryos (907210. 77 ± 145520. 77,186224. 33 ± 103308. 00, P< 0.05), and it was significantly lower than those of 2-cell embryos, molura and blastocyst (1563422. 54 ± 224666. 51, 1697626. 25±176999. 53 and 1752301. 29± 101146. 64, P<0. 05). mtDNA copy number of parthenogenetic expanded blastocyst was 2812545. 67± 156819. 31, which was the highest value of all different developmental embryos. In conclusion, mito-chondrial distribution and mtDNA copy number changes could differ in the developmental process of parthenogenetic embryos.关键词
猪卵母细胞/孤雌激活/线粒体分布/mtDNA拷贝数Key words
porcine oocyte/parthenogenetic/mitochondrial distribution/mtDNA copy number分类
农业科技引用本文复制引用
成文敏,魏红江,潘伟荣,信吉阁,黄言,曾养志..猪孤雌激活早期胚胎线粒体分布及mtDNA拷贝数变化的研究[J].中国畜牧兽医,2012,39(1):127-131,5.基金项目
国家自然科学基金项目(30800785) （30800785）
云南省教育厅科学研究基金重点项目(0920042). （0920042）
