中国畜牧兽医2012，Vol.39Issue(1)：136-141,6.
猪肉质性状功能基因在野猪群体内的变异研究
Research on Variation from Pork Quality Trait Function Gene in Wild Boar Population
摘要
Abstract
The aim of this research was to understand the wild pig population genetic variation in different meat quality traits function genes. In this study, we chose MC4R gene (D298N G>A), RYRl gene (c. 1843 OT) ,PRKAG3 gene (1849 G> A) and IGF2 gene(3072 G> A) mutation sites and used for genotyping by PCR-RFLP and PCR-SSCP methods using 72 DNA from wild boar experimental animals. The results showed that in the RYRl c. 1843 C＞T mutation site, CC genotype was the wild boar population dominant genotype, allele C was dominant gene within the wild boar population, no found TT genotype and RYRl c. 1843 OT mutation site was not Hardy-Weinberg equilibrium(P<0. 01 and P<0. 05). The MC4i? Gene D298N G> A point mutation, GG genotype was the wild boar population dominant genotype, allele G was dominant gene within the wild boar population. With PCR-BsrB I-RFLP used for detection wild boar population, GG genotype was dominant genotype,allele G was dominant gene, no found AA genotype. No variation was detected in wild boar populations for JGF2 gene 3072 G>A mutation. GG genotype was dominant genotype in wild boar population by sequencing validated. Furthermore, the population genetic analytic results showed that these mutation sites were Hardy-Weinberg equilibrium(P>0. 05). It was concluded that the wild boar population had lower genetic variation and diversity and consistent with the breed characteristic.关键词
野猪/肉质性状/多态性Key words
wild boar/meat quality traits/polymorphisms分类
农业科技引用本文复制引用
杨广礼,郭艳,闫永峰,韩进诚,付冬丽,欧阳,施传信,徐珂..猪肉质性状功能基因在野猪群体内的变异研究[J].中国畜牧兽医,2012,39(1):136-141,6.基金项目
河南省科技攻关项目(092102110088 （）
2010B230007) （）
商丘师范学院青年自然科学基金(2010QN19). （2010QN19）
