中国畜牧兽医2012，Vol.39Issue(1)：168-172,5.
牦牛大肠杆菌的分离鉴定及系统发育分析
Isolation, Identification and Phylogenetic Analysis of E.coli from Yaks
摘要
Abstract
The objective of this paper is to study the genetic evolutionary relationship of E. Coli from yaks and identify their genetic relationship with human and other species of animals . A total of 60 anal swabs from healthy adult yaks in Ganzi andAba prefecture were collected and inoculated in MaConkey agar. Suspected colonies were further identified by morphological observation, cultural character, biochemical assays and 16S rRNA PCR detection, and 49 strains of E. Coli were finally obtained. The 16S rRNA of 9 E. Coli strains was cloned and sequenced, and the phylogenetic analysis showed that the homology was high up to 98. 8% to 99. 9% among yak-origin strains, while the homology with other strains in GenBank was 94. 0% to 100%. The results in this paper indicate that the yak-origin E. Coli strains have close genetic distance with strains from cattle, and have closer distance with the strains from human, pig, duck and chicken, and have farther distance with the caprine strains, in which two strains are gathered to one cluster with human enterohemorrhagic E. Coli (EHEC) O157 : H7 and Shigel-la, and these two strains may be the potential pathogenic bacteria for human being.关键词
大肠杆菌/牦牛/16SrRNA/系统发育分析Key words
E. Coli/yak/16S rRNA/phylogenetic analysis分类
农业科技引用本文复制引用
任丽倩,于学辉,宋定州,甄康娜,覃蔚,王远微..牦牛大肠杆菌的分离鉴定及系统发育分析[J].中国畜牧兽医,2012,39(1):168-172,5.基金项目
国家自然科技资源平台建设项目(2005DKA21205-11) （2005DKA21205-11）
国家科技支撑计划(2009BADB4B01) （2009BADB4B01）
中央高校基本科研业务费科研平台建设项目(11NPT03). （11NPT03）
