中国畜牧兽医2012，Vol.39Issue(1)：225-227,3.
贝类折光马尔太虫PCR检测方法的建立
Development of a Polymerase Chain Reaction Assay for Detection of Marteilia re fringens in Shellfish
摘要
Abstract
According to the gene sequences in GenBank of Marteilia refringens ? One pair of specific primers were designed for amplifying the specific fragments of Marteilia refringens. The reaction parameters were optimized to develop the polymer-ase chain reaction method for detection of Marteilia refringens. The 478 bp long specific DNA fragment of Marteilia refringens was amplified, but not from other pathogenic such as Perkinsus sp. , Haplosporidium sp. , Aeromonas hydrophila, Pseudomonas fluorescens , Vibrio parahaemolyticu , Vibrio alginolyticu , and Vibrio fluviaUs by this PCR. The sensitivity results showed that as little as 1 pg DNA of Marteilia refringens was detected by this PCR. 119 Oyster samples of Guangxi coastal were detected by this PCR. As a result, the positive rate was 1. 68%, It suggested that Marteilia refringens existed in the cultivated shellfish in south China and the PCR method could be used as a sensitive tool to detect Marteilia refringens in clinical samples.关键词
贝类/折光马尔太虫/PCRKey words
shellfish/Marteilia refringens/PCR分类
农业科技引用本文复制引用
谢丽基,谢芝勋,庞耀珊,刘加波,邓显文,谢志勤..贝类折光马尔太虫PCR检测方法的建立[J].中国畜牧兽医,2012,39(1):225-227,3.基金项目
国家百千万人才工程人选专项资金项目(945200603) （945200603）
广西特聘专家专项经费(2011B020) （2011B020）
广西科技攻关项目(桂科攻0630001-3M). （桂科攻0630001-3M）
