中国畜牧兽医2012，Vol.39Issue(2)：28-31,4.
马泰勒虫病PCR检测方法的建立和应用
Development and Application of PCR Assay to Detect Theileria equi
摘要
Abstract
To establish a PCR detection method of Theileria equi, the 18S rRNA gene recently discovered was shown to be species-specific. A pair of primers was designed to specifically amplify a 531 bp fragment. And the T. Equi, T. Equi and B. Caballimixture, B. Caballi, T. Uilenbergi, T. Sinensis, T. Annulata, T. Ovis, T. Luwenshun, T. Sergenti were tested by PCR. While T. Equi genome templates were amplified that different concentrations diluted in order to determine the sensitivity of the experiment. 45 equine blood samples were detected by the PCR and microscopic. The PCR result of specificity assay showed that one references T. Equi and mixed with B. Caballi could be detected by the PCR test, but no amplification was observed when 'other 7 bacterial species B. Caballi, T. Uilenbergi, T. Sinensis, T. Annulata, T.ovis, T. Luwenshuni, T. Sergenti tissue were detected. And the sensitivity result showed that the minimum dose of T. Equi that could be detected by PCR assay was 10-13, 45 clinical samples, from horses farm in China, doubtedly infected with T. Equi were tested by PCR. Relevance ratio of T. Equi was 17. 78% (8/45) by PCR and microscopic examination found that only 8. 89% (4/45). Between the coincidence rate was 100%. This research established T. Equi PCR detection method is definitely a good detection method.关键词
马泰勒虫/PCR诊断方法/建立/应用Key words
Theileria equi/ PCR diagnosis method/ development/ application分类
农业科技引用本文复制引用
罗金,刘光远,谢俊仁,田占成,党根生..马泰勒虫病PCR检测方法的建立和应用[J].中国畜牧兽医,2012,39(2):28-31,4.基金项目
甘肃省科技重大专项(092NKDA031). （092NKDA031）
