中国畜牧兽医2012，Vol.39Issue(2)：31-34,4.
奶牛乳腺上皮细胞核蛋白质组双向电泳方法的建立
Establishment of Two-dimensional Electrophoresis Method of Bovine Mammary Epithelial Cell Nucleus Proteomics
摘要
Abstract
The resolution of 2-DE map is highly altered due to different experimental conditions, a 2-DE map with high resolution can be obtained via the optimization of key experimental conditions for a definite proteome sample. To establish an effective separation system of 2-DE for the proteome of bovine mammary epithelial cell nucleus, 2-DE method was researched on the standard experimental condition with an optimization for each important experimental parameter. The numbers of protein spots, resolution and contaminating strands in 2-DE maps were determined. A 2-DE separation system for proteome of bovine mammary epithelial cell nucleus proteins was successfully established.关键词
双向电泳/蛋白质组/奶牛乳腺上皮细胞/细胞核蛋白质Key words
2-DE/ proteomics/ bovine mammary epithelial cells cell nucleus proteins分类
生物科学引用本文复制引用
刘荣,高学军,李庆章,黄建国,陆黎敏,骆超超,金鑫,王佳丽..奶牛乳腺上皮细胞核蛋白质组双向电泳方法的建立[J].中国畜牧兽医,2012,39(2):31-34,4.基金项目
国家重点基础研究发展(973)计划(2011CB100804). （973）
