中国畜牧兽医2012，Vol.39Issue(2)：40-42,3.
基于TaqMan探针的虎制品实时荧光定量PCR的特异性鉴定
Real-time Quantitative RT-PCR Assay for Specific Identification of Panthera tigris Materials
摘要
Abstract
Primers and probes were designed based on the conservative sequence of cytochondriome cytochrome b, a realtime quan quantitative RT-PCR assay was developed for indentification of Panthera tigris. The recombinant plasmid containing the target sequence was constructed to detect the sensitivity and prepare the standard curve. The real-time RT-PCR assay had a detection limit of 50 copies/μL, and with a dynamic range of detection between 5 × 105 to 5 × 101 copies /μL. The primers and probe were specific for Panthera tigris' DNA and did not react with other non- Panthera tigris ' DNA. This real-time RT-PCR assay with high sensitivity, specificity and accuracy is considered to be a powerful tool for the rapid indentification and quantification of Panthera tigris.关键词
虎/实时荧光定量PCR/TaqMan探针Key words
Panthera tigris/ real-time quantitative RT-PCR/ TaqMan probe分类
农业科技引用本文复制引用
张太翔,凌宗帅,赵立娜,袁涛,徐彪,梁成珠,刘文鹏,孙军,张艺兵..基于TaqMan探针的虎制品实时荧光定量PCR的特异性鉴定[J].中国畜牧兽医,2012,39(2):40-42,3.基金项目
国家质量监督检验检疫总局课题资助(2009IK004). （2009IK004）
