中国畜牧兽医2012，Vol.39Issue(2)：48-51,4.
蜥蜴利什曼原虫在小鼠体内的分布及增殖研究
Distribution and Proliferation of Leishmania tarentolae in BALB/c Mice
摘要
Abstract
BALB/c mice inoculated with Leishmania tarentolae parasites which express fluorescent protein by intraperitoneal routes. Frozen sections of their organs were stained with fluorescent dye and observed by fluorescence microscopy. The results showed that Leishmania tarentolae were observed in heart, liven spleen, lung and kidney of BALB/c mice. In addition, Leishmania real-time quantitative PCR method was established successfully and used to detect Leishmania tarentolae in BALB/c mice. These results indicated that the proliferation curve of Leishmania tarentolae in BALB/c mice was wavy in 13 days. The results for the study of Leishmania tarentolae will provide the theoretical basis for pathogenesis, vaccine development in humans and animals.关键词
蜥蜴利什曼原虫/荧光染色/实时荧光定量PCRKey words
Leishmania tarentolae/fluorescent staining/real-time quantitative PCR分类
农业科技引用本文复制引用
李琳,靳朝,夏志平,马丽敏,任秀梅,何叶,李晔,商利民,胡乐鹏,薛慧亮,李丹..蜥蜴利什曼原虫在小鼠体内的分布及增殖研究[J].中国畜牧兽医,2012,39(2):48-51,4.基金项目
国家高技术研究发展计划(2009AA10Z4483730). （2009AA10Z4483730）
