中国畜牧兽医2012，Vol.39Issue(2)：174-177,4.
锦鲤溃疡病病原菌的分离及16S rRNA鉴定
Isolation of Ulcer Disease Pathogen from Infected Ornamental carp (Cyprinus carpio L.) and Identification of the Strain's 16S rRNA
摘要
Abstract
The study was carried out to search for the pathogenic factor causing Ornamental carp ulcer disease. A pathoge-netic bacterial strain CL0901 was isolated from naturally infected Ornamental carp (Cyprinus carpio L. ) in Beijing. The strain was identified according to its physiological and biochemical properties, and the sequence analysis of 16S rRNA gene. The drugs sensitivity was detected with Kirby-Bauer's agar diffusion method. Based on the result of 16S rRNA sequence analysis, the strain CL0901 shares 99. 9% sequence identity with the type strain ATTCC 35624T of Aeromonas veronii. Combining with bacteria observation, physiological and biochemical characteristics, the pathogenetic bacterial strain CL0901 was identified as Aeromonas veronii. Drug sensitive tests showed that isolation was sensitive to Neomycin, Piperacillin, Levofloxacin, Nor-floxacin, Gentamicin, Amikacin, Tazobactam, Pefloxacin, Lomefloxacin and Tobramycin.关键词
锦鲤/溃疡病/生理生化特性/16S rRNA基因/药敏试验Key words
Ornamental carp sulcer disease/physiological and biochemical characteristics/ sequences analysis of 16S rRNA gene/drug sensitive test分类
农业科技引用本文复制引用
姜娜,马志宏,李铁梁,邢薇,罗琳..锦鲤溃疡病病原菌的分离及16S rRNA鉴定[J].中国畜牧兽医,2012,39(2):174-177,4.基金项目
北京市科技项目(D09060500430000) （D09060500430000）
北京市农林科学院青年基金. （）
