中国畜牧兽医2012，Vol.39Issue(2)：221-224,4.
不同脱钙液对兔膝关节组织切片染色的影响
The Influence of Different Decalcifying Fluid on the Section Staining ofArticular Joint Tissue of Rabbit Knee
英永 1王韶艳 1王凤乾 1胡建廷 1李艳 1屈卉锦 1李莉 1邱波1
作者信息
- 1. 山东省医药工业研究所药物安全性评价中心山东省化学药物重点实验室,山东济南250033
- 折叠
摘要
Abstract
To investigate the effect of different decalcifying fluid on the color of articular joint tissue section of rabbit knee, to aid at the histological evaluation of hyaline cartilage and distribution of fibrocartilage polysaccharide. Prepare New Zealand rabbits,after the fully fixation of the whole rabbit knee joints in 10% neutral formalin, the tissues were washed with running water,then decalcified with 4 different decalcifying solutions (10% HNO3 decalcifying solutions,3% HNO3 decalcifying solutions, modified AF decalcifying solutions and modified 30% hydrochloric acid). The tissue sections were stained with hematox-ylin-eosin (HE),alcian blue and safranin-O.and observed under a microscope to evaluate the morphology of chondrocytes and the cartilage matrix. Results:?The effects of HE staining: first is 3% HNO3 decalcifying solutions group,next is modified 30% hydrochloric acid group,once again is 10% HNO3 decalcifying solutions and modified AF decalcifying solutions group. ?The effect of alcian blue and safranin-0 staining in 3% HNO3 decalcifying solutions group and modified AF decalcifying solutions group were better than other groups.关键词
膝关节/脱钙液/染色Key words
knee joint/ decalcifying fluid/ staining分类
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英永,王韶艳,王凤乾,胡建廷,李艳,屈卉锦,李莉,邱波..不同脱钙液对兔膝关节组织切片染色的影响[J].中国畜牧兽医,2012,39(2):221-224,4.
