中国畜牧兽医2012，Vol.39Issue(2)：225-227,3.
日本乙型脑炎病毒小鼠脑内分布研究
Japanese Encephalitis Virus Distribution in the Brain of Mice
摘要
Abstract
To localize and detect the distribution of Japanese encephalitis virus (JEV) in the brain of experimental mouse, we established JEV infection model in mouse and produced paraffin sections using brain tissue. We established indirect immunohis-tochemical method with monoclonal antibodies of JEV to localize and detect the antigen. Experiment results showed that the method had a high sensitivity and specificity, it demonstrated that JEV infect neurons selectively, the antigen expression were mainly in the brainstem, ganglia and cortex.关键词
日本乙型脑炎病毒/间接免疫酶组化/抗原分布Key words
apanese encephalitis virus/ indirect immunohistochemistry/ antigen distribution分类
农业科技引用本文复制引用
马丽敏,李丹,靳朝,夏志平,李琳,张宁,胡乐鹏,李晔,何叶,任秀梅,李志萍,薛慧亮..日本乙型脑炎病毒小鼠脑内分布研究[J].中国畜牧兽医,2012,39(2):225-227,3.基金项目
国家高技术研究发展计划(863计划)课题(2009AA10Z4483730). （863计划）
