中国畜牧兽医2012，Vol.39Issue(3)：23-27,5.
SHIV-KB9病毒中国恒河猴细胞适应株的制备
The Propagation and Evaluation of SHIV-KB9 Adapative Virus Stock in Chinese Original Rhesus Macaques
摘要
Abstract
To propagate SHIV-KB9 virus stock via passaged in Chinese-original monkey PBMCs and realize viral replication and immunological trauma in the monkey after inoculation intravenously with 10 fold dilution of SHIV-KB9. Infectious virus was generated by ligating pSHIV-KB9 5' and pSHIV-KB9 3', and transfecting CEMxl74 cells. The peripheral blood mononu-clear cells(PMBCs) from Chinese-original rhesus macaque were cocultured with the supernatant of transfection and the virus stock was collected when the viral replication reached the peak area. The variation of env gene was analized, so did the viral loads and tissue culture infective dose (TCID50)with TZM-bl cells. Three Chinese-origin rhesus monkeys were infected with this batch of SHIV-KB9 intravenously with 10 fold dilution and the viral loads in plasma and the change of CD4+ and CD8+ T lymphocytes were analysed during the whole infection. In this study, totally 95 mL virus stock was propagated in monkeys PBMCs and without any variation about the gpl20 sequence of env gene. The viral loads was 2. 678 X 105 copies/mL and had 3. 16 X 103 TCID50 in 1 mL of cell-free SHIV-KB9 stock. 3 rhesus macaques were infected and presented severe and rapid depletion of CD4 ' cells during acute phase. This panel of SHIV-KB9 adapted in Chinese-original rhesus monkeys' PBMCs were highly infectious and suitable for the animal modle.关键词
SHIV-KB9/中国恒河猴/外周血单核淋巴细胞/半数感染量Key words
SHIV-KB9/CCR5-Specifc/ Chinese-original rhesus monkeys/PBMCs/TCID50分类
医药卫生引用本文复制引用
丛喆,陶真,王卫,陈霆,金光,姚南,苏爱华,魏强..SHIV-KB9病毒中国恒河猴细胞适应株的制备[J].中国畜牧兽医,2012,39(3):23-27,5.基金项目
“十二·五”国家科技重大专项(2012ZX10004-501). （2012ZX10004-501）
