中国畜牧兽医2012，Vol.39Issue(3)：33-36,4.
Vero E6细胞中B23.1基因的扩增及其原核表达
Amplification and Prokaryotic Expression of B23.1 Protein Gene in Vero E6 Cell
摘要
Abstract
A fragment containing t323.1 protein gene of Veto E6 cell was amplified by reverse transcriptase-polymerase chain reaction(RT-PCR)using the upper/lower primers each including the recognition sequence for BamH I and Xho I re spectively.The results showed that the ORF of B23.1 gene was composed of 901 hp,deletion and insertion were not found.By digestion with restriction enzymes BamH I and Xho I,the B23.1 gene were cloned into pOEX-6p-1 vector to construct recombinant plasmid named pGEX-6p-B23.1.The recombinant plasmid was transformed into E.Coil BL21(DE3)and induced with IPTG.The results of SDS-PAGE assay showed that the protein was largely expressed,the GST-tagged protein was puri-fied.Western blotting analysis showed that the target protein had a good reactivity with B23 monoclonal antibody(F/louse).关键词
Vero E6细胞/B23.1基因/重组蛋白/反应活性Key words
Vero E6 cell B23.1 gene/recombinant protein/reactivity分类
生物科学引用本文复制引用
吕茂杰,陈建飞,时洪艳,冯力..Vero E6细胞中B23.1基因的扩增及其原核表达[J].中国畜牧兽医,2012,39(3):33-36,4.基金项目
中央级公益性科研院所基本科研业务费(200805). （200805）
