中国畜牧兽医2012，Vol.39Issue(3)：92-94,3.
副猪嗜血杆菌16S rRNA基因的克隆及序列分析
Cloning and Sequence Analysis of 16S rRNA Gene for Haemophilus parasuis in Swine
摘要
Abstract
Haemophilus parasuis were identified by PCR from Hunan province isolates, and used 16S rRNA sequences to reconstruct their phylogenetic relationship. The partial 16S rRNA was amplified from each H. parasuis, and sequences were a-ligned using the ClustalX 1. 81. MP and NJ trees of 16S rRNA were constructed using the software Phylip 3. 67 version 4. 0 and Mega version 4. 0, and ML tree was also constructed using Puzzle version 5. 2. Sequence homology analysis was performed using the Megalign program of the software DNAStar version 5. 0. The results showed that the lengths of 16S rRNA sequences were 783 bp. The constructed phylogenetic tree revealed that the Hunan isolates and the H. parasuis serovar 05 available in GenBankwere clustered in the same clade. The results of the present study provided foundation for further studies of molecular epidemiology of H. parasuis and for diagnosis of the resultant disease.关键词
副猪嗜血杆菌/16S rRNA/种系发育关系Key words
H. parasuis/16S rRNA/phylogenetic relationship分类
生物科学引用本文复制引用
王祝荣,卢寄军,张进..副猪嗜血杆菌16S rRNA基因的克隆及序列分析[J].中国畜牧兽医,2012,39(3):92-94,3.基金项目
长沙科技计划一般项目(K0902144-21)资助. （K0902144-21）
