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产角蛋白酶菌株的筛选、鉴定和发酵特性的初步研究

郭刚 楚杰 王君高 刘可春

中国畜牧兽医2012,Vol.39Issue(3):125-129,5.
中国畜牧兽医2012,Vol.39Issue(3):125-129,5.

产角蛋白酶菌株的筛选、鉴定和发酵特性的初步研究

Screening,Identification of a Strain with High Keratinase Production and the Primary Study of its Characterization of Fermentation

郭刚 1楚杰 1王君高 2刘可春1

作者信息

  • 1. 山东轻工业学院食品与生物工程学院,山东济南250353
  • 2. 山东省科学院生物研究所,山东济南250014
  • 折叠

摘要

Abstract

A strain with high keratinolytic activity was obatained by casein plate firstly and feather as the sole carbon sourceand nitrogen source in submerged medium secondly. The strain S6 was identified as Bacillus licheniformis based on its morphologic and physicochemical characteristics. After fermenting 72 h in submerged culture, the Bacillus Licheniformis S6 demonstrated highest keratinolytic activity and maximum of soluble protein, 186. 8 U/mLand 160. 87 jig/mL,respectively, which expresses that Bacillus subtilis S6 has a promising prospect in the degradation process of feather.

关键词

筛选/鉴定/地衣芽孢杆菌/角蛋白酶活力/可溶性蛋白

Key words

screen/identification/Bacillus licheniformis /keratinolytic activity/soluble protein

分类

生物科学

引用本文复制引用

郭刚,楚杰,王君高,刘可春..产角蛋白酶菌株的筛选、鉴定和发酵特性的初步研究[J].中国畜牧兽医,2012,39(3):125-129,5.

中国畜牧兽医

OA北大核心CSTPCD

1671-7236

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