中国畜牧杂志2012，Vol.38Issue(3)：1-5,59,6.
GPR54基因多态性与小梅山猪产仔数的关联分析
Polymorphism of GPR54 Gene and Its Relationship with Litter Size in Xiaomeishan Pigs
摘要
Abstract
The aim of present study was to detect the polymorphism of GPR54 gene and investigate the relationship between GPR54 gene and litter size in pigs. The polymorphisms of GPR54 genes were detected by PCR-SSCP, PCR-RFLP and DNA sequencing methods in 218 sows of three pig populations, including Xiaomeishan pigs, Fengjing pigs and Large White pigs, and the effects of genotypes of GPR54 gene loci on reproductive records of 616 litters of Xiaomeishan piglets were analyzed by the least square analysis. There were three polymorphisms occurring in exonl (Primer 1), intron2 (Primer 4) and exon5 (Primer 8), in which one polymorphism (P1) resulted in amino acid change (Leu35Pro), the others were in genetic linkage. At P1 locus, BB genotype had 0.69 and 1.65 (P<0.01) piglets more than AB and AA genotypes in Xiaomeishan pigs after two parities. The TNB and NBA in BB genotype were significantly higher than those in AB and AA genotypes in all parities (P<0.01). At P4/P8 locus, the heterozygous had higher TNB and NBA than the homozygous had. The results suggested that the BB genotype at P1 locus was the favorable genotype used as a genetic marker in assisted selection of Xiaomeishan pigs.关键词
GPR54/PCR-SSCP/产仔数/小梅山猪Key words
GPR54/ PCR-SSCP/ litter size/ xiaomeishan pigs分类
农业科技引用本文复制引用
吴井生,张跟喜,戴国俊,叶兰,王金玉..GPR54基因多态性与小梅山猪产仔数的关联分析[J].中国畜牧杂志,2012,38(3):1-5,59,6.基金项目
江苏高校优势学科建设工程资助项目 （）
江苏省农业三项工程项目(sx〈2009〉25、sx〈2010〉244) （sx〈2009〉25、sx〈2010〉244）
江苏省畜禽技术改进项目(2009〈46〉) （2009〈46〉）
