中国畜牧杂志2012，Vol.38Issue(3)：22-25,4.
绵羊去透明带卵母细胞体细胞核移植研究
Somatic Cell Nuclear Transfer of Zona-free of Ovine Oocytes
摘要
Abstract
Nuclear transfer were compared in this study in order to establish a simply nuclear transfer procedure, including oocytes maturation culture time, the time of oocytes exposed to UV, electric fusions, activated items and develpomental method. When oocytes were cultured for 18-19 h, the attaching rates of the first poly body and first poly body were appropriate for oocyte maturation. Electric fusions were performed in 1.9 Kv/cm,the fusion rates were 88.2%. Enucleated zona-free oocytes and somatic cells were fused to reconstruct embryos, and the embryos were activated by Ionomycin, the cleavage and blastocyst rates were 82.1% and 10.4%. Reconstructed embryos were activated by the same methods after cultured in the WOWs (only pressed), there was no significant difference compared with that in four-well dish. When zona-free ovine oocytes was irradiated by UV (1/4) beyond 10s, most of ovine reconstruct embryos can not cleave to blastocyst after activation. The results indicated the zona-free cloning method might be easier to adopt than conventional cloning procedure for users, and was an easy procedure.关键词
卵母细胞/去透明带/体细胞核移植/绵羊Key words
oocyte/ zona-free/ somatic cell nuclear transfer/ ovine分类
农业科技引用本文复制引用
杜晨光,张小宇,郭凤英,李国俊,郭志凯..绵羊去透明带卵母细胞体细胞核移植研究[J].中国畜牧杂志,2012,38(3):22-25,4.基金项目
内蒙人才基金(B62038) （B62038）
内蒙古高等学校科学研究项目(NJ10060) （NJ10060）
