中国烟草科学2012，Vol.33Issue(1)：23-26,4.DOI:10.3969/j.issn.1007-5119.2012.01.005
烟草抗病毒相关R基因RNAi表达载体的构建
Construction of Tobacco Virus Resistance-related R Genes RNAi Expression Vectors
摘要
Abstract
Gene silence mediated by RNA interference has been shown to be a highly rapid and effective method for breeding resistance to virus and analyzing plant functional genomics. Building of RNAi expression vector and being transformed into tobacco can obtain virus resistance tobacco materials and provide a new method for identification of tobacco functional genes in a large-scale. The partial sequence of R genes with specific banding site attB were amplified by PCR technology, primers were designed according to the sequences obtained from Nicotiana tomentosiformis sequencing. Using GATEWAY technology, the RNAi expression vector of R gene was constructed successfully by insered the partial sequence of R genes into the vector of pH7GWIWG2(I).关键词
烟草/病毒病/R基因/RNAi/GATEWAY技术Key words
tobacco/ virus/ R gene/ RNAi/ GATEWAY technology分类
轻工纺织引用本文复制引用
杨帆,王元英,李凤霞,孙玉合,王鲁,杨爱国,苏振刚,李元元,赵百英,刘慧..烟草抗病毒相关R基因RNAi表达载体的构建[J].中国烟草科学,2012,33(1):23-26,4.基金项目
国家烟草专卖局重点科技项目“烟草cDNA文库构建及重要基因表达谱研究”(110200701021) （110200701021）
“烟草突变体库创制与功能基因组学研究”(110200701022) （110200701022）
“优质抗病烤烟新品种选育及育种技术研究”(110201002002) （110201002002）
