中国烟草学报2012，Vol.18Issue(1)：85-91,7.DOI:10.3969/j.issn.1004-5708.2012.01.015
烟草优质品种叶片全长cDNA文库的构建和质量分析
Construction of full-length cDNA library from leaf of quality tobacco varieties and its characterization
摘要
Abstract
Leaves of variety Cuibi 1 from different periods were used as material to extract total RNA to study molecular mechanism of quality leaf tobacco. cDNA was synthesized by SMART technique and a full- length cDNA library was constructed by modified method of clontech technology. Results indicated that the primary library storage capacity was 3. 85 x 106, the recombinant rate was 100% with insert fragment concentrated in 750-2000 bp. Twenty-five clones were randomly selected and sequenced and subjected to preliminary bioinformatics analysis. Ten of the sequences had been reported in tobacco while eleven with full-length sequence and twenty sequences with function annotation. Results also indicated that the library was of high quality, which lay the foundation for further cloning and screening target genes from the library.关键词
烟草/叶片/SMART技术/cDNA文库Key words
tobacco/ leaf/ SMART technology/ cDNA library分类
农业科技引用本文复制引用
曾建斌,陈顺辉,陈华,贺小彦,姜宝杰,张冲,蔡铁城,庄伟建..烟草优质品种叶片全长cDNA文库的构建和质量分析[J].中国烟草学报,2012,18(1):85-91,7.基金项目
国烟科(2006)371,闽烟科(2008) 13,福建省作物分子与细胞生物学重点实验室项目(2008J1033) （2006）
