中国油料作物学报2012，Vol.34Issue(1)：101-105,5.
油棕EST序列中SSR的分布特征分析
Analysis of SSR characteristics in EST sequences of oil palm (Elaeis guineensis Jacg.)
摘要
Abstract
In order to make full use of the EST resources in the present public database to develop the EST -SSR primers, in this study, 39 227 ESTs of oil palm (Elaeis guineensis Jacg. ) from GenBank database were downloaded and analyzed, resulting in 15 716 non - redundant sequences with total length about 7 977. 734 kb. 699 SSRs were discovered, the frequency of these EST - SSRs was 4.45% and the mean distance was 11.41 kb in non - redundant ESTs. Among them the dinucleotide and trinucleotide repeats were dominant types, accounting for 38.48% and 25. 32% respectively. AG/CT was repeated most in all nucleotide repeat motifs, accounting for 27. 47% . Blast analysis showed that 44. 95% of SSR - ESTs were homologous with functional gene in non - redundant protein sequences databases ( Nr database). The homology with known functions from Vitis vinifera had the greatest proportion and reached up to 4.42%. Simultaneously, the functional classification of 54 SSR -ESTs as made by GO system, of which 46 were found in the Nr and EST database, and the other 8 showed no homology. These ESTs can be divided into 18 functional subclasses, and the functional items and SSR -ESTs relevant to binding presented higher quantity than all other functional subclasses. The function of other 580 ESTs was not researched.关键词
油棕/EST/SSRKey words
Oil palm/ EST/ SSR分类
农业科技引用本文复制引用
吴春太,陈青,刘锐,李维国..油棕EST序列中SSR的分布特征分析[J].中国油料作物学报,2012,34(1):101-105,5.基金项目
国家天然橡胶产业体系(nyhyzx07-033-1) （nyhyzx07-033-1）
海南省自然科学基金(809031) （809031）
