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紫外分光光度法测定弱视明口服液中总多糖含量

梁学政 唐勇琛 吕建伟 陈惠红 陆玉婷

中国药业2012,Vol.21Issue(7):17-18,2.
中国药业2012,Vol.21Issue(7):17-18,2.

紫外分光光度法测定弱视明口服液中总多糖含量

Determination of Total Polysaccharide in Ruoshiming Mixture by UV-Spectrophotometry

梁学政 1唐勇琛 1吕建伟 1陈惠红 1陆玉婷2

作者信息

  • 1. 广西壮族自治区柳州市中医院,广西柳州,545001
  • 2. 广西中医学院研究生学院2008级研究生,广西南宁,530001
  • 折叠

摘要

Abstract

Objective To establish the method for determination of total polysaccharide in Ruoshiming Mixture by UV - spectrophotometry. Methods The total polysaccharide in Ruoshiming Mixture was tested at the wavelength of 490 nm with UV - spectrophotometry by using glucose as standard substance. Results The regression equation was A = 15. 571 X + 0. 026v r = 0. 999 8 ). The linear range of glucose was 0. 008 08 - 0. 048 48 g/L( r = 0. 999 8, n = 5 ). The average recovery was 99. 51% , RSD was 1. 26% ( n = 6 ). Conclusion The method is simple, accurate, reproducible and is suitable for content determination of Ruoshiming Mixture.

关键词

弱视明口服液/总多糖/紫外分光光度法/苯酚-硫酸法/含量测定

Key words

Ruoshiming Mixture/total polysaccharide/UV - spectrophotometry/phenol - sulfuric colorimetric analysis/content determination

分类

医药卫生

引用本文复制引用

梁学政,唐勇琛,吕建伟,陈惠红,陆玉婷..紫外分光光度法测定弱视明口服液中总多糖含量[J].中国药业,2012,21(7):17-18,2.

中国药业

OACSTPCD

1006-4931

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