中国肿瘤生物治疗杂志2012，Vol.19Issue(1)：17-21,5.DOI:10.3872/j.issn.1007-385X.2012.01.003
IL-12与IL-18联合刺激对树突状细胞分泌的exosome活性的影响
Effect of stimulation with combined IL-12 and IL-18 on exosomes secreted by dendritic cells
摘要
Abstract
Objective: To observe the effect of stimulation with combined IL-12 and IL-18 on exosomes secreted by dendritic cells (DCs) , and so as to facilitate exploring high-efficiency DC derived exosome(Dex) cancer vaccines. Methods: DCs induced from human peripheral blood mononuclear cells (PBMCs) were stimulated with IL-12 + IL-18,IL-12 or IL-18 for the combined group,IL-12 group and IL-18 group respectively; and no stimulant for the control group. Dex was extracted from DCs. The expressions of HLA-DR and CD83 in Dex were detected by Western blotting. CD54, CD80 and CD86 expressions were determined by flow cytometry. The T cell proliferation stimulated by Dex was detected with MTT method. IFN--y in the T cell supernatant was detected by ELISA. Results; HLA-DR and CD83 proteins were expressed in Dex of all the four groups. The molecule expressions for combined group were CD54(323.67 ±44.06 i? 246.17 ± 31.91, 236.33 + 33. 87, 167.67 ± 28.73, P < 0.05), CD80(406.37 ±39.18 vs 331.67 ±36.15, 335.67 ±41.38, 260.00±35.58, P<0.05), and CD86(390.50±38.06 vs 314.33 ± 36.64, 319.00±33.10, 246.83+30.55, P<0.05), which were higher than those in the IL-12 group, IL-18 group and control group. The T cell proliferation in the combined group (1.98 ±0.31 ?s 1.55 ±0.23, 1.57 ±0.21, 1.10 ±0.18, P <0.05) washigher than that in the IL-12 group, IL-18 group and control group. IFN-Υ secreted by T cells in the combined group (436.67 ± 61.8 is 295.04 ±40.25, 358.18 ±55.77, 225.00 ±36.44, P <0.05) was higher than that in the IL-12 group, IL-18 group and control group. Conclusion: Stimulation of DCs with combined IL-12 and IL-18 can enhance Dex express CD54, CD80 and CD86, promote Dex-induced T cell proliferation and increase IFN-Υ secretion by T cells.关键词
树突状细胞/exosome/IL-12/IL-18/T细胞/IFN-γKey words
dendritic cell/ exosome/ IL-12/ IL-18/ T cell/ IFN-Υ分类
医药卫生引用本文复制引用
张在云,李希德,魏玮,叶兰,丛萍,潘祥林..IL-12与IL-18联合刺激对树突状细胞分泌的exosome活性的影响[J].中国肿瘤生物治疗杂志,2012,19(1):17-21,5.基金项目
山东省自然科学基金资助项目(No.Y2007C111) （No.Y2007C111）
