中国肿瘤生物治疗杂志2012，Vol.19Issue(1)：35-39,5.DOI:10.3872/j.issn.1007-385X.2012.01.006
EPHA2基因腺病毒感染树突状细胞诱导CTL对胶质瘤细胞的杀伤作用
Cytotoxic effect of CTLs elicited by dendritic cells infected with adenovirus containing EPHA2 gene on glioma cells
摘要
Abstract
Objective;To explore the cytotoxic effect of cyctotoxic T lymphocytes (CTLs) induced by dendritic cells (DCs) modified by EPHA2 gene on U251 glioma cells, and to provide new ways for glioma immune therapy. Methods; DCs originated from the HLA-A2 + PBMCs were infected with recombinant adenovirus containing EPHA2 full length cDNA, and the DC vaccine modified by EPHA2 gene was prepared. The expression of EPHA on DCs was detected by Western blotting and FACS. HLA-A2 + PBMCs were stimulated by the DC vaccine in vitro. The specificity CTL activity induced by rAd-EPHA2-DCs and the cytotoxicity on HLA-A2 + U251 glima cells were detected by enzyme-linked immunospot assay (EliS-POT) and standard 51 Cr release experiment. Results: The DC vaccine modified by EPHA2 gene was successfully prepared, and EPHA2 protein was effectively expressed. Compared to DCs infected with rAd-LacZ and PBS groups, DCs infected with rAd-EPHA2 stimulated CTL activity effectively ([187 ±21] vs [12 ±4], [18 ±5]; P <0.01) and the CTLs induced by rAd-EPHA2-DCs produced cytotoxicity effect on U251 cells obviously ([45.7+6.8]% vs [7.1 ±4.5]%, P<0.01), and did not cause the cytotoxicity of its own lymphocytes. Conclusion; The DC vaccine modified by EPHA2 gene can stimulate the specificity CTL response effectively, and can cause cytotoxicity on glioma U251 cells obviously.关键词
EPHA2基因/树突状细胞/胶质瘤Key words
EPHA2 gene/ dendritic cell/ glioma分类
医药卫生引用本文复制引用
陈宏颉,袁邦清,王守森,郑兆聪,高进喜,王如密..EPHA2基因腺病毒感染树突状细胞诱导CTL对胶质瘤细胞的杀伤作用[J].中国肿瘤生物治疗杂志,2012,19(1):35-39,5.基金项目
福建省自然科学基金资助项目(No.2006J0373) （No.2006J0373）
