中国肿瘤生物治疗杂志2012，Vol.19Issue(1)：72-76,5.DOI:10.3872/j.issn.1007-385X.2012.01.013
pGPU6/GFP/Neo-hTERT-shRNA对结直肠癌SW480细胞裸鼠移植瘤的抑制
Inhibition of pGPU6/GFP/Neo-hTERT-shRNA on colorectal cancer SW480 cell xenograft in nude mice
摘要
Abstract
Objective:To investigate the treatment effect of recombinant plasmid pGPU6/GFP/Neo-hTERT-shRNA targeting hTERT gene on human colorectal cancer SW480 cell xenograft in nude mice. Methods: Human colorectal cancer SW480 cells were subcutaneously implanted under the skin of the right armpit to establish nude mice model of colorectal cancer, after the tumors grew to a definite size. The mice were randomly divided into three groups; normal saline ( NS group), PGPU6/GFP/Neo-NC-shRNA group ( NC-shRNA group) and PGPU6/GFP/Neo-hTERT-shRNA group (hTERT-shRNA group). After each group was treated for 6 consecutive times, the growth status of the tumor was observed, tumor volume was measured, tumor growth curve was drawn,tumor tissue morphology was observed with H-E staining, the expression of hTERT protein in the tumors was detected by immunohistochemistry, cell apoptosis was inspected by terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling ( TUNEL) , and the expression of hTERT mRNA was checked by RT-PCR. Results: The growth of tumor volume became slower in hTERT-shRNA group than did that in NS group and NC-shRNA group. Compared with NS group and NC-shRNA group, the tumor cell morphology changed obvious-ly and the number of apoptotic cells increased significantly in the transplanted tumor tissues in hTERT-shRNA group ([36.3 ±5.05]% i? [5.25 ±1.06]%, [6.95 ±1.07]% ,P<0.01). Compared with NS group and NC-shRNA group, the expression of hTERT protein was significantly inhibited in hTERT-shRNA group( [ 171.42 ± 30.94] is [ 146. 89 ±21.43 ], [ 137. 35 ±25.49 ], P <0.01). Compared with NS group and NC-shRNA group, the expression of hTERT mRNA was significantly inhibited in hTERT-shRNA group (0.39 ± 0.09 i? 0. 81 ± 0.34, 0.75 ± 0.21, P < 0. 05). Conclusion: Recombi-nant plasmid pGPU6/GFP/Neo-hTERT-shRNA promotes apoptosis of implanted human colorectal cancer by down-regulating the expression of hTERT mRNA and hTERT protein in tumor tissues, thus inhibiting the growth.关键词
RNA干扰/hTERT基因/结直肠癌/裸鼠/凋亡Key words
RNA interference/ hTERT gene/ colorectal cancer/ nude mice/ apoptosis分类
医药卫生引用本文复制引用
蔡艳玲,罗小玲,葛连英,刘爱群,谢裕安..pGPU6/GFP/Neo-hTERT-shRNA对结直肠癌SW480细胞裸鼠移植瘤的抑制[J].中国肿瘤生物治疗杂志,2012,19(1):72-76,5.基金项目
广西自然科学基金资助项目(No.2010GXNSFA013238) （No.2010GXNSFA013238）
广西卫生厅重点课题资助项目(No.Z200971). （No.Z200971）
